Name of Targeting Construct:
Targeting Frequency (i.e. number of positive ES cells per screening):
(Please submit screening assay results that
demonstrate correct targeting gene of interest)
Source of ES cells:
The targeted ES cells are mycoplasma-free.
The ES cells MUST be shown to be mycoplasma free before we will inject
them. Please call us to discuss options for
The targeted ES cell line has a normal karyotype.
If your cells have not been tested for normal karyotype, we highly recommend
that you do so prior to submitting them for injection. Dr. Chih-lin Hsieh
can do this test (Chih-Lin.Hsieh@med.usc.edu).
Charge: $350/cell line. You will need to supply the equivalent of a T25
flask of 50% confluent cells.
Type of Targeted ES Cell for Injection:
Targeted ES cell (129) microinjection for Knock-out/inTargeted ES cell (C57BL) microinjection for Knock-out/in
Mouse Protocol #
= Total $
If outside USC, you may use a PO, including CHLA and Doheny Inst.