Flow Cytometry and Immune Monitoring Core (FCIM)
The mission of the Flow Cytometry and Immune Monitoring Core is to provide advanced flow cytometry,
cell sorting and immune monitoring capabilities and services for advancing basic and
translational research with an option for full-service technical support for clinical
immunobiology and immune monitoring correlative studies needed for the pathogenesis,
treatment, and prevention of cancer.
The Flow Cytometry and Immune Monitoring Core comprises the following Cores:
||Flow Cytometry -
Services include state-of-the-art technology to selectively phenotype and isolate
specific populations of cells. Using fluorescent probes and transgenic proteins,
researchers monitor several aspects of their cell populations, including phenotype,
cell viability, proliferation, cell cycle and cell activation. Additionally, cell
purification by electrostatic cell sorting enables cutting-edge research on low frequency cells.
||Immune Monitoring -
Services include blood and tissue processing and banking, equipment and technical
assistance for functional immune assessment (multi-parameter flow cytometry on PBMC,
ELISpot assays, proliferation assays, cytotoxicity assays, antibody and cytokine
profiling by luminex multiplexing technology), molecular –based quantitative PCR
assays, and option for full service technical support and project management for
clinical trial immune monitoring. Assays and technical support are also available
to support pre-clinical translational in vivo animal model research.
Gregor Adams, Ph.D.
Lora Barsky (Flow Cytometry)
Diane Da Silva, Ph.D., M.S. (Immune Monitoring)
W. Martin Kast, Ph.D. (Immune Monitoring)