University of Southern California
USC Norris Comprehensive Cancer Center
If you are a patient, please contact Keck Medical Center of USC at 800-USC-CARE.

Flow Cytometry and Immune Monitoring Core (FCIM)

The mission of the Flow Cytometry and Immune Monitoring Core is to provide advanced flow cytometry, cell sorting and immune monitoring capabilities and services for advancing basic and translational research with an option for full-service technical support for clinical immunobiology and immune monitoring correlative studies needed for the pathogenesis, treatment, and prevention of cancer.
The Flow Cytometry and Immune Monitoring Core comprises the following Cores:

Flow Cytometry - Services include state-of-the-art technology to selectively phenotype and isolate specific populations of cells. Using fluorescent probes and transgenic proteins, researchers monitor several aspects of their cell populations, including phenotype, cell viability, proliferation, cell cycle and cell activation. Additionally, cell purification by electrostatic cell sorting enables cutting-edge research on low frequency cells.
Immune Monitoring - Services include blood and tissue processing and banking, equipment and technical assistance for functional immune assessment (multi-parameter flow cytometry on PBMC, ELISpot assays, proliferation assays, cytotoxicity assays, antibody and cytokine profiling by luminex multiplexing technology), molecular –based quantitative PCR assays, and option for full service technical support and project management for clinical trial immune monitoring. Assays and technical support are also available to support pre-clinical translational in vivo animal model research.

Core Directors:
Rong Lu, Ph.D.

Core Supervisors:
Jeffry Boyd (Flow Cytometry)
Diane Da Silva, Ph.D., M.S. (Immune Monitoring)
W. Martin Kast, Ph.D. (Immune Monitoring)