University of Southern California
USC Norris Comprehensive Cancer Center

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If you are a patient, please contact Keck Medical Center of USC at 800-USC-CARE.

Pronuclear Injection Transgenic Mouse Order Form

  • We will harvest 150-200 eggs (B6D2F1) and inject into the pronucleus of fertilized ova with DNA prepared and purified by the investigator (protocols available upon request). We ask that the investigator supply a photo of the gel of the purified DNA for injection. Injected eggs that survive to the 2 cell stage will be implanted into pseudopregnant females.
  • We will pay for the maintenance of foster mothers and pups up to three weeks from date of birth. During that time we expect the PI to (1) Genotype the mice; (2) Transfer the mice to the PI's vivarium protocol. We will charge double for the mice left on the Core protocol after three weeks.
  • In general, the injection will be repeated until at least 2 transgenic pups are obtained (except the cases in which the transgenic causes embryonic lethality). Transgenic status refers only to DNA integration as assessed by Southern blot. We can't guarantee expression of an RNA or protein product from the integrated DNA.

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Contact Person Ordered:
Email      Fax

Name of Injection Construct:

Size of transgene fragment (kb):

Plasmid vector employed to propagate transgene: 

Restriction enzymes used to excise transgene: 

Description of transgene (promoter, expressed sequence, 3' UTR): 

Method of DNA purification: 

If yes: Donors Stud Male

If yes, explain:

Mouse Protocol #

= Total $    

If outside USC, you may use a PO, including CHLA and Doheny Inst.

Payment Method:

USC Account # or PO:
Organization   (optional)
Grant #   (optional)
Human Stem Cell research? Under penalty of law, please honestly indicate if this order is for non-Federally approved Human Stem Cell research? Federal regulations require these projects not be subsidized by federal funds. If yes, additional costs may apply.  
Invoice Mailing Address (POs)
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*To copy order completed emails to your PI/Lab Manager, contact us.