University of Southern California
USC Norris Comprehensive Cancer Center

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If you are a patient, please contact Keck Medical Center of USC at 800-USC-CARE.

ES Cell Injection "Knock-out/in" Rat Order Form

  • We will inject 2-3 independent targeted ES cell clones (provided by the investigator) into blastocysts.
  • In total, 120 blastocysts will be injected and 10 embryo transfers will be performed.
  • We do not guarantee germline transmission. However, our experience indicates that if the ES cells are of good quality, then the above procedure has a strong probability of producing at least two germline chimeras.
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Contact Person Ordered:
Email      Fax

Name of Targeting Construct:

Targeting Frequency (i.e. number of positive ES cells per screening):
(Please submit screening assay results that demonstrate correct targeting gene of interest)

Source of ES cells:

The ES cells MUST be shown to be mycoplasma free before we will inject them.  Please call us to discuss options for mycoplasma testing.

If your cells have not been tested for normal karyotype, we highly recommend that you do so prior to submitting them for injection.  Dr. Chih-lin Hsieh can do this test (  Charge: $350/cell line.  You will need to supply the equivalent of a T25 flask of 50% confluent cells.

Type of Targeted ES Cell for Injection:

Rat Protocol #

= Total $    

If outside USC, you may use a PO, including CHLA and Doheny Inst.

Payment Method:

USC Account # or PO:
Organization   (optional)
Grant #   (optional)
Human Stem Cell research? Under penalty of law, please honestly indicate if this order is for non-Federally approved Human Stem Cell research? Federal regulations require these projects not be subsidized by federal funds. If yes, additional costs may apply.  
Invoice Mailing Address (POs)
Enter Address
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*To copy order completed emails to your PI/Lab Manager, contact us.