University of Southern California
USC Norris Comprehensive Cancer Center
If you are a patient, please contact Keck Medical Center of USC at 800-USC-CARE.

ES Cell Gene Targeting for Rats

  • Advice on vector design.
  • Electroporation (TG/KO provides DA rES cells).
  • Selection of 200-400 ES cell colonies.
  • Customer performs PCR or Southern blot for screening.
  • Additional services available for additional charges.
  • For injection of ES cells into blastocysts, please see our "Knock-Out" Rat order form 

All order forms now require you to be logged in before placing orders. Please click here to log in or click here to create a USC Norris website account.

Contact Person Ordered:
Email      Fax

Vector Name:

Gene Targeted:

Selection Marker Genes:

We will need map of the targeting vector, screening strategy and copy of a southern blot showing detection of expected bands by the 5’ and 3’ probes. Restriction enzyme to be used screening of clones.

DNA fragment concentration as determined by OD260: 

Require Screening Service?       

Restriction enzyme to be used for linearized DNA: 

Note: Picture of DNA undigested and digested required.

The ES cell gene “knock-out” or “knock-in” requires the full cooperation between the investigator and the gene targeting core facility, these are complex experiments whose success cannot be guaranteed.

= Total $    

If outside USC, you may use a PO, including CHLA and Doheny Inst.

Payment Method:

USC Account # or PO:
Organization   (optional)
Grant #   (optional)
Human Stem Cell research? Under penalty of law, please honestly indicate if this order is for non-Federally approved Human Stem Cell research? Federal regulations require these projects not be subsidized by federal funds. If yes, additional costs may apply.  
Invoice Mailing Address (POs)
Enter Address
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*To copy order completed emails to your PI/Lab Manager, contact us.