Selection Marker Genes:
will need map of the targeting vector, screening strategy and copy of a
southern blot showing detection of expected bands by the 5’ and 3’ probes.
Restriction enzyme to be used screening of clones.
DNA fragment concentration as determined by OD260:
Restriction enzyme to be used for linearized DNA:
Note: Picture of DNA undigested and digested required.
The ES cell gene “knock-out” or “knock-in” requires the full cooperation between
the investigator and the gene targeting core facility, these are complex
experiments whose success cannot be guaranteed.
Select the type of service:ES Cell Gene Targeting (129)Cassettes deletion (Cre or Flp recombination in ES cells with selection markers)
= Total $
If outside USC, you may use a PO, including CHLA and Doheny Inst.