The storage of mouse embryos in liquid nitrogen offers a safe way to preserve
lines with potential future use but no current use. This also saves
facility space and the expense associated with keeping a breeding colony.
Another advantage is that during the process of embryo collection, most
pathogenic organisms are excluded. For cryopreservation of a mouse line,
the P.I. coordinates the receipt of at least 10 males ages 2.5 - 6 months of
the desired strain under the P.I.'s animal care program number. The Core
acquires Wt B6D2F1 females (P.I. must provide the females if a specific
background is required) and schedules the freezing 200 or more fertilized eggs
from each line in straws. As a quality control, the Core will thaw a
straw of embryos along with each frozen line and will culture them to the
= Total $
If outside USC, you may use a PO, including CHLA and Doheny Inst.