Equipment

• RareCyte + Leica RXm: Analyses all nucleated cells (no enrichment). Automated high throughput 4-channel staining (Leica) followed by high-content scanning and image analysis for CTC identification and robotic micropipette recovery (RareCyte). Use for CTC enumeration, protein and RNA -IF/FISH, and single CTC recovery for DNA/RNA profiling
  


• CellSearch (Menarini/SBI): Automated, FDA-cleared immuno-affinity capture, enumeration and immunofluorescence (IF) protein profiling
  


• Parsortix (Angle): Size-based microfluidic CTC enrichment for gene expression profiling
  


• Parylene-C slot microfilters (USC/Caltech): Size-based live CTC capture for immunofluorescence (IF) and live cell assays (e.g. telomerase activity)
  


• ClearCell FX (Clearbridge Biomedics): Size-based inertial focusing CTC capture for enumeration, immunofluorescence and genomic profiling
  


• DepArray – 2nd generation (Silicon Biosystems/SBI): Dielectrophoretic cage capture, 6-channel IF, and single CTC recovery for DNA/RNA profiling
  


• LiquidBiopsy (Cynvenio): Immuno-affinity capture for targeted sequencing of CTCs and matched plasma